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  • Title: [Mechanisms of heat shock proteins inhibiting C2C12 cell apoptosis induced by hydrogen peroxide].
    Author: Xiao WM, Jiang BM, Shi YZ.
    Journal: Zhong Nan Da Xue Xue Bao Yi Xue Ban; 2004 Feb; 29(1):6-10. PubMed ID: 16136992.
    Abstract:
    OBJECTIVE: To explore the mechanisms of C2C12 cell apoptosis induced by hydrogen peroxide (H2O2) which is inhibited by heat shock proteins. METHODS: The expression of heat shock proteins in mouse embryonic myogenic cell line, C2C12 cell,was induced by heat shock response. C2C12 cell apoptosis induced by 0.5 mmol/L hydrogen peroxide (H2O2) was determined by Hoechst 33258 staining. The activities of caspase -3,8,9 were assayed by caspase colorimetric assay kit and Western-blotting. The release of cytochrome C from mitochondria was observed by Western-blotting of cell mitochondria and cytosol fractions. RESULTS: The expression of HSP70 and alphaB-crystallin in C2C12 cell significantly increased at 24 hour after the heat shock response. Heat shock response could inhibit the release of cytochrome c from mitochondria to cytoplasm,the activation of caspase -3,8,9 and the subsequent apoptosis induced by H2O2 in C2C12 cell. CONCLUSION: HSPs can inhibit the C2C12 cell apoptosis through interference with the activation of both mitochondrial and death receptor pathways, which can provide new clues for the prevention of cardiovascular diseases.
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