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  • Title: [Increased expression of peroxisome proliferator-activated receptor-gamma in endothelial cells of spontaneously hypertensive rats].
    Author: Qi YX, Niu XL, Wei J, Wang NP.
    Journal: Zhong Nan Da Xue Xue Bao Yi Xue Ban; 2004 Oct; 29(5):525-8. PubMed ID: 16137038.
    Abstract:
    OBJECTIVE: To evaluate the relationship between hypertension and the expression level of PPAR-gamma in endothelial cells (ECs) of spontaneously hypertensive rats (SHR). METHODS: PPAR-gamma protein expression in ECs of 4-week and 16-week SHR was detected by immunohistochemistral technique and analyzed by image acquiring and analysis system, and age matched Wistar-Kyoto rats (WKY) were used as controls. The aortic ECs of different-aged rats were primarily cultured, and the protein and mRNA level of PPAR-gamma in cultured ECs (< or =3 passages) were detected by Western blotting and reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: The blood pressure of 4-week SHR was slightly higher than those of 4-week WKY (P < 0.05). The protein and mRNA levels of PPAR-gamma in 4-week SHR were also higher than that of 4-week WKY, but the differences had no statistical significance (P > 0.05). Compared with the 16-week WKY, the blood pressure of 16-week SHR greatly increased (P < 0.01), and the protein and mRNA levels of PPAR-gamma in ECs from 16w SHR were approximately 1.5 times higher than those of the age matched WKY (P < 0.01). The protein and mRNA levels of PPAR-gamma in ECs from 16-week SHR were 2.5 times higher than those of 4-week SHR (P < 0.01), while in 16-week WKY it was only 1.5 times higher than those of 4-week WKY (P < 0.01). Conclusion In accordance with the hypertension progress, the protein and mRNA levels of PPAR-gamma in SHR can significantly increase.
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