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  • Title: [Analysis of TCR Vbeta clonotypes of T lymphocytes infiltrating in the joints of rats with collagen-induced arthritis].
    Author: Zhang XM, Zhao WM, Li Y, Liu ZL.
    Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2005 Sep; 21(5):538-40. PubMed ID: 16143051.
    Abstract:
    AIM: To investigate the TCR Vbeta clonetypes of T lymphocytes infiltrating in the synovium of rats with collagen induced arthritis (CIA). METHODS: Type II collagen was used to induce arthritis in Lewis rats. CD4(+) and CD8(+) T lymphocytes in peripheral blood of the rats were analyzed by flow cytometry (FCM). Total RNA was extracted from rat joint tissues and TCR Vbeta clonotypes were identified by RT-PCR and single-strand conformation polymorphism (SSCP). Splenic lymphocytes from CIA rats were transferred into normal Lewis rats. TCR Vbeta clonotypes of T lymphocytes infiltrating in the joints of the recipient rats were also analyzed by RT-PCR/SSCP. RESULTS: FCM analysis of the T lymphocytes indicated that CD8(+) T lymphocytes decreased in CIA rats and the CD4(+)/CD8(+) T lymphocytes ratio was >2, which suggested that there was a disproportion of the T lymphocyte subsets in CIA rats. RT-PCR/SSCP analysis showed that TCR Vbeta5.2 and TCR Vbeta8.2 clonotypes predominated in the T lymphocytes infiltrating in the joints of CIA rats. T lymphocytes infiltrating in the joints of the recipient rats displayed the same TCR Vbeta clonotypes as those in the donor rats. CONCLUSION: CIA rats may have a limited number of dominant TCR Vbeta clonotypes of T lymphocytes accumulating in the synovium, of which TCR Vbeta5.2 and 8.2 predominate.
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