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  • Title: [Preparation, purification and identification of the rabbit antibody against extracellular region of mLAIR-1].
    Author: Zhang Y, Zhuang R, Jin BQ, Ouyang WM.
    Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2005 Sep; 21(5):595-7. PubMed ID: 16143065.
    Abstract:
    AIM: To express and purify the fusion protein of the extracellular region of murine leukocyte-associated Immunoglobulin-like receptor-1 (mLAIR-1) molecule with Fc fragment of human IgG and to prepare the rabbit antibody against mLAIR-1 extracellular region. METHODS: The eukaryotic expression vector pIg/3c-mLAIR-1 was constructed. Rabbits were immunized with the fusion protein purified from the culture medium of mLAIR-1-Fc vector-transfected CHO cells with protein A column. The rabbit antibody against mLAIR-1 extracellular region were purified through Sepharose-4B affinity column coupled with mLAIR-1-Fc. The specificity of the antibody was characterized by indirect fluorescence staining and FCM analysis. RESULTS: The mLAIR-1-Fc fusion protein was expressed and purified. The rabbit antibody against the extracellular region of mLAIR-1 molecule was obtained, which could react with both natural mLAIR-1 molecule on P388D1 cells and recombinant mLAIR-1 molecule on transfected CHO cells. CONCLUSION: The rabbit antibody against the extracellular region of mLAIR-1 is prepared successfully, which may provide a useful tool for studying the structure and function of mLAIR-1.
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