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  • Title: Direct molecular detection of nucleic acids by fluorescence signal amplification.
    Author: Ho HA, Doré K, Boissinot M, Bergeron MG, Tanguay RM, Boudreau D, Leclerc M.
    Journal: J Am Chem Soc; 2005 Sep 14; 127(36):12673-6. PubMed ID: 16144416.
    Abstract:
    An integrated PCR-free DNA sensor, which combines a sequence-specific receptor, an optical polymeric transducer, and an intrinsic fluorescence amplification mechanism, is reported. This sensor is based on the different conformations adopted by a cationic polythiophene when electrostatically bound to ss-DNA or ds-DNA, and on the efficient and fast energy transfer between the resulting fluorescent polythiophene/ds-DNA complex and neighboring fluorophores attached to ss-DNA probes. This molecular system allows the detection of only five molecules in 3 mL of an aqueous solution, or 3 zM, in 5 min. Moreover, this work demonstrates, for the first time, the direct detection of single nucleotide polymorphisms (SNPs) from clinical samples in only a few minutes, without the need for nucleic acid amplification.
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