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  • Title: The influence of a membrane environment on the structure and stability of a prokaryotic potassium channel, KcsA.
    Author: Encinar JA, Molina ML, Poveda JA, Barrera FN, Renart ML, Fernández AM, González-Ros JM.
    Journal: FEBS Lett; 2005 Sep 26; 579(23):5199-204. PubMed ID: 16150445.
    Abstract:
    The lack of a membrane environment in membrane protein crystals is considered one of the major limiting factors to fully imply X-ray structural data to explain functional properties of ion channels [Gulbis, J.M. and Doyle, D. (2004) Curr. Opin. Struct. Biol. 14, 440-446]. Here, we provide infrared spectroscopic evidence that the structure and stability of the potassium channel KcsA and its chymotryptic derivative 1-125 KcsA reconstituted into native-like membranes differ from those exhibited by these proteins in detergent solution, the latter taken as an approximation of the mixed detergent-protein crystal conditions.
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