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  • Title: Signaling pathway in nerve growth factor induced histamine release from rat mast cells.
    Author: Stempelj M, Ferjan I.
    Journal: Inflamm Res; 2005 Aug; 54(8):344-9. PubMed ID: 16158335.
    Abstract:
    OBJECTIVE AND DESIGN: We investigated a signal transduction pathway involved in NGF induced histamine secretion from mast cells. We compared this mechanism with the exocytosis induced by basic secretagogue compound 48/80. MATERIALS AND METHODS: Isolated rat peritoneal mast cells were obtained from male Wistar rats. Histamine release was assayed spectrofluorometrically. RESULTS: We found that tyrosine kinase inhibitor genistein, phospholipase C (PLC) inhibitor U-73122, phosphatidylinositol 3-kinase (PI-3 kinase) inhibitor wortmannin, protein kinase C (PKC) inhibitors, staurosporine and GF109203X, but not MAP kinase inhibitors, SB203580 and PD98059, reduce histamine secretion in NGF provoked mast cell degranulation. In compound 48/80 mediated degranulation, we confirmed only the involvement of tyrosine kinase and PLC, but not PI-3 kinase, PKC and MAP kinases. CONCLUSIONS: Our results indicate that release of histamine from mast cells after stimulation with NGF is regulated by tyrosine kinase, PLC, PI-3 kinase and PKC, but not by MAP kinases. This biochemical pathway differs from that provoked by compound 48/80.
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