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  • Title: Instrumentation for the breath-by-breath determination of oxygen and carbon dioxide based on nondispersive absorption measurements.
    Author: Arnoudse PB, Pardue HL, Bourland JD, Miller R, Geddes LA.
    Journal: Anal Chem; 1992 Jan 15; 64(2):200-4. PubMed ID: 1616125.
    Abstract:
    This paper describes the development and evaluation of instrumentation for the breath-by-breath determination of oxygen and carbon dioxide in respiratory gases. The method is based on nondispersive absorption and uses the 145-nm absorption band for detection of oxygen and the 4.3-micron band for detection of carbon dioxide. A xenon discharge lamp with a sharp band at 147 nm was chosen as the source for the determination of oxygen, and a carbon dioxide discharge lamp with a sharp band at 4.3 micron was chosen for determination of carbon dioxide. A vacuum photodiode was used as the detector for oxygen, and a photoconductive cell with a built-in interference filter was used for detection of carbon dioxide. Plots of absorbance (A) vs concentration (C, %) were linear for oxygen and were nonlinear for carbon dioxide. Typical least-squares calibration equations were A = 0.020C + 0.02 for oxygen (0-100%) and A = 0.0012C2 + 0.050C + 0.008 for carbon dioxide (0-8%). Comparisons of computed (y) vs prepared (x) values for the concentrations given above were linear for both gases, yielding y = (1.00 +/- 0.01)x - 0.13 +/- 0.73 for oxygen and y = (1.07 +/- 0.02)x - 0.04 +/- 0.06 for carbon dioxide. The standard deviations were 1.2% at 50% oxygen and 1.5% at 4% carbon dioxide. Records are presented to illustrate breath-by-breath monitoring of these gases in a healthy subject.
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