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  • Title: [Determination of cholesteryl palmitate in Oviductus Ranae by HPLC].
    Author: Wang YS, Jiang DC, Meng Q, Wang ES.
    Journal: Zhongguo Zhong Yao Za Zhi; 2005 Jul; 30(13):990-1. PubMed ID: 16161425.
    Abstract:
    OBJECTIVE: To develop a new method for the determination of cholesteryl palmitate in Oviductus Ranae. METHOD: A HPLC method was set up, using Zorbax Silica column and cyclohexane-diethyl ether (40:1) as mobile phase with a flow rate of 1.0 mL x min(-1), and the UV detection wavelength was 203 nm. RESULT: The calibration curve was linear over the range of 0.60-8.92 microg (r = 0.9997), the average recovery of the method was 98.4%. RSD 1.8% (n = 6). CONCLUSION: The results showed that method was reliable and accurate.
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