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  • Title: [Detection of quorum-sensing pathway and construction of LuxS gene deletion mutants of group B Streptococcus].
    Author: Ou YQ, Ma WL, Liu CH, Shi R, Zheng WL.
    Journal: Di Yi Jun Yi Da Xue Xue Bao; 2005 Sep; 25(9):1135-9. PubMed ID: 16174581.
    Abstract:
    OBJECTIVE: To detect the possible presence of AI-2 quorum-sensing pathway and construct group B Streptococcus (GBS) mutants with deletion of LuxS gene related to quorum-sensing pathway. METHOD: V. harveyi BB170 was employed as the reporter strain to detect AI-2 pathway in GBS, and identification of LuxS homologous gene in GBS type V strain 2603 was performed by software-based analysis. LuxS gene deletion mutant Delta LusX was then constructed in GBS by means of allelic exchange and verified by Southern hybridization analysis. RESULTS: A component in the secretions of GBS could induce bioluminescence activity in the reporter strain, suggesting the presence of AI-2 quorum-sensing pathway in GBS. luxS homologous gene was detected in GBS and LuxS gene deletion mutants was successfully constructed in GBS Ia 515 and V 2603 strains. CONCLUSION: This study establishes a bacterial model for studying the role of LuxS molecule in AI-2 quorum-sensing pathway in GBS and provides new insights into virulence regulation mechanism of GBS.
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