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  • Title: [Production and characterization of a group of monoclonal antibodies to human transitional cell carcinoma].
    Author: Guo YL.
    Journal: Zhonghua Wai Ke Za Zhi; 1992 Nov; 29(12):777-80, 799-800. PubMed ID: 1618045.
    Abstract:
    A panel of specific mouse monoclonal antibodies, BIU-H4, BIU-H6, BIU-H9, was obtained by immunization with BIU-87 cells (a transitional cell carcinoma cell line of the bladder), which was blocked by the serum of mouse anti-human lymphocyte. Detailed descriptions were given concerning their reactivity patterns by ABC-ELISA and IIF assays against various cells. They showed positive reactions with transitional cell carcinoma of the bladder and negative with normal bladder. BIU-H6 did not react with any of the other normal or malignant tissues. The subclass of immunoglobulin for BIU-H4,6 was IgG1, for BIU-H9 is IgG2 alpha. The molecular weight of the antigen recognized by BIU-H6,9 is 102 KD.
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