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  • Title: Enzyme association with lipidic Langmuir-Blodgett films: interests and applications in nanobioscience.
    Author: Girard-Egrot AP, Godoy S, Blum LJ.
    Journal: Adv Colloid Interface Sci; 2005 Nov 30; 116(1-3):205-25. PubMed ID: 16181605.
    Abstract:
    This review presents the recent advances in the achievement of organized proteo-lipidic nanostructures based on Langmuir-Blodgett technology and their potential applications in the nanobioscience area. By using the self-assembled properties of amphiphilic biomolecules at the air-water interface, the Langmuir-Blodgett (LB) technique offers the possibility to prepare ultrathin layers suitable for biomolecule immobilization at the molecular level. This review will provide a general overview of the enzyme association with preformed Langmuir-Blodgett films in connection with their potential applications in biosensing device developments, and then introduce the design of a new functionalised biomimetic nanostructure with oriented recognition site. The potential applications of such an organized proteo-lipidic nanostructure for biocatalysis investigations of an immobilised enzyme in a biomimetic situation and for the development of bioelectronic devices are finally discussed.
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