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  • Title: [Molecular characteristics of three new isolates of Japanese encephalitis virus in Fujian Province].
    Author: Chen D, Wang HY, Fu SH, Song H, Deng J, Yang YL, Liang GD.
    Journal: Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi; 2005 Mar; 19(1):5-8. PubMed ID: 16201460.
    Abstract:
    OBJECTIVE: To analyze the genotype of newly isolated Japanese encephalitis viruses in Fujian province and the characteristics of amino acid sequence in the E gene. METHODS: PrM and E segments of newly isolated Japanese encephalitis viruses were amplified by RT-PCR, the PCR products were cloned into T vector for sequencing. Phylogenetic analysis was carried out by PHYLIP program. RESULTS: Newly isolated Japanese encephalitis viruses belonged to genotype III, the nucleotide and amino acid of E gene showed high homology to vaccine strain SA14-14-2, but had some difference in some domains. CONCLUSION: Newly isolated viruses in Fujian province belonged to Japanese encephalitis virus genotype III. E protein of the isolates showed some differences as compared with vaccine strains.
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