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  • Title: Single multiplex PCR assay to identify simultaneously the six categories of diarrheagenic Escherichia coli associated with enteric infections.
    Author: Vidal M, Kruger E, Durán C, Lagos R, Levine M, Prado V, Toro C, Vidal R.
    Journal: J Clin Microbiol; 2005 Oct; 43(10):5362-5. PubMed ID: 16208019.
    Abstract:
    We designed a multiplex PCR for the detection of all categories of diarrheagenic Escherichia coli. This method proved to be specific and rapid in detecting virulence genes from Shiga toxin-producing (stx(1), stx(2), and eae), enteropathogenic (eae and bfp), enterotoxigenic (st II and lt), enteroinvasive (vir F and ipa H), entero-aggregative (aaf II), and diffuse adherent (daa E) Escherichia coli in stool samples.
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