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  • Title: Characterization of the Drosophila melanogaster mitochondrial proteome.
    Author: Alonso J, Rodriguez JM, Baena-López LA, Santarén JF.
    Journal: J Proteome Res; 2005; 4(5):1636-45. PubMed ID: 16212416.
    Abstract:
    We have combined high-resolution two-dimensional (2-D) gel electrophoresis with mass spectrometry with the aim of identifying proteins represented in the 2-D gel database of Drosophila melanogaster mitochondria. First, we purified mitochondria from third instar Drosophila larvae and constructed a high-resolution 2-D gel database containing 231 silver-stained polypeptides. Next, we carried out preparative 2-D PAGE to isolate some of the polypeptides and characterize them by MALDI-TOF analysis. Using this strategy, we identified 66 mitochondrial spots in the database, and in each case confirmed their identity by MALDI-TOF/TOF analysis. In addition, we generated antibodies against two of the mitochondrial proteins as tools for characterizing the organelle.
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