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  • Title: An enhanced method for peptides sequencing by N-terminal derivatization and MS.
    Author: Noga MJ, Lewandowski JJ, Suder P, Silberring J.
    Journal: Proteomics; 2005 Nov; 5(17):4367-75. PubMed ID: 16220530.
    Abstract:
    An improved method for peptide sequencing based on acetylation/deuteroacetylation in conjunction with ESI MS is introduced. Derivatization with a 1:1 mixture of acetic anhydride and deuterated acetic anhydride incorporates a stable isotope label into the analyzed molecule. This approach has been initially applied to FAB. Using MS/MS, the technique provides a fast, highly sensitive and reliable determination of the primary structure of unknown peptides. This procedure labels N-terminal fragments formed during MS/MS analysis, resulting in a simplification and faster interpretation of the spectra. The performance of the method has been tested with several synthetic peptides and applied to an efficient sequencing of the peptide map, using a nano-scale LC coupled on-line to a tandem mass spectrometer.
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