These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Screening of protein crystallization conditions on a microfluidic chip using nanoliter-size droplets.
    Author: Zheng B, Roach LS, Ismagilov RF.
    Journal: J Am Chem Soc; 2003 Sep 17; 125(37):11170-1. PubMed ID: 16220918.
    Abstract:
    Protein crystallization is a major bottleneck in determining tertiary protein structures from genomic sequence data. This paper describes a microfluidic system for screening hundreds of protein crystallization conditions using less than 4 nL of protein solution for each crystallization droplet. The droplets are formed by mixing protein, precipitant, and additive stock solutions in variable ratios in a flow of water-immiscible fluids inside microchannels. Each droplet represents a discrete trial testing different conditions. The system has been validated by crystallization of several water-soluble proteins.
    [Abstract] [Full Text] [Related] [New Search]