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  • Title: An Alu-based, MGB Eclipse real-time PCR method for quantitation of human DNA in forensic samples.
    Author: Nicklas JA, Buel E.
    Journal: J Forensic Sci; 2005 Sep; 50(5):1081-90. PubMed ID: 16225212.
    Abstract:
    The forensic community needs quick, reliable methods to quantitate human DNA in crime scene samples to replace the laborious and imprecise slot blot method. A real-time PCR based method has the possibility of allowing development of a faster and more quantitative assay. Alu sequences are primate-specific and are found in many copies in the human genome, making these sequences an excellent target or marker for human DNA. This paper describes the development of a real-time Alu sequence-based assay using MGB Eclipse primers and probes. The advantages of this assay are simplicity, speed, less hands-on-time and automated quantitation, as well as a large dynamic range (128 ng/microL to 0.5 pg/microL).
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