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  • Title: Crystallization and preliminary crystallographic analysis of the N-terminal domain of PriA from Escherichia coli.
    Author: Sasaki K, Ose T, Tanaka T, Mizukoshi T, Ishigaki T, Maenaka K, Masai H, Kohda D.
    Journal: Biochim Biophys Acta; 2006 Jan; 1764(1):157-60. PubMed ID: 16226927.
    Abstract:
    PriA, a DEXH-type DNA helicase, binds specifically to the 3' end of DNA through its N-terminal domain, and is a candidate sensor protein that recognizes arrested DNA replication forks in bacteria. We crystallized an N-terminal fragment of PriA in the absence and the presence of oligonucleotides to elucidate the structural basis for the specific recognition of the 3' terminus of DNA.
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