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  • Title: [Research on differential display genes of tolerant-damage induced by trichloroethylene].
    Author: Wei QZ, Zhuang ZX, Yuan JH, Huang HY, Li X, Yang X, Geng L.
    Journal: Wei Sheng Yan Jiu; 2005 Jul; 34(4):403-6. PubMed ID: 16229258.
    Abstract:
    OBJECTIVE: To observe the differentially expressed genes of the human normal liver cell (L-02) induced by low concentration of trichloroethylene (TCE). METHODS: The dose-effect relation of TCE toxicity was analyzed by means of MTT. 5 micromol/L was chosen as low concentration while 40 micromol/L as high concentration. Then L-02 were treated with low concentration, high concentration, pretreated with low concentration then attacked with high concentration of TCE respectively. Fluorescence differential display polymerase chain reaction (Fluoro DD-PCR) was used to search differentially expressed genes of the different treatment groups of TCE. Results 51 differential expressed strap were found, 11 differential straps have been cloned and sequenced. 9 of them were known gene, while 2 of them were new genes. CONCLUSION: The differential straps would be identified to provide scientific base for research deeply the mechanism of low concentration TCE inducing adaptive response of L-02.
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