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  • Title: [Alteration and its significance to expression of aquaporin-4 in cultured neonatal rat astrocytes in the model of hypoxic damage].
    Author: Fu XM, Yao YJ, Yang Z, Xiang L, Gao J.
    Journal: Sichuan Da Xue Xue Bao Yi Xue Ban; 2005 Sep; 36(5):641-4. PubMed ID: 16235526.
    Abstract:
    OBJECTIVE: To investigate the expression of aquaorin-4 (AQP4) in the cultured neonatal rat primary astrocytes during hypoxia and during reoxygenation after hypoxia. METHODS: The astrocytes were randomly assigned to the hypoxia group (the astrocytes were exposed to hypoxia for 3 h, 6 h, 12 h and 24 h respectively), the reoxygenation group (after exposure to 12 h hypoxia, the astrocytes were returned to normoxic cultures for 3 h, 6 h, 12 h and 24 h respectively), and the normoxic group (control group). AQP4 mRNA expression in astrocytes of all groups was detected by Real Time PCR, and AQP4 protein expression was determined by immunofluorescence. RESULTS: The AQP4 mRNA and protein expressions significantly decreased with the time of hypoxia, and during reoxygenation, the decreased expressions were gradually up-reguated. CONCLUSION: It is likely that the decreased expression of AQP4 is associated with brain edema and in such brain edema conditions AQP4 may play a prominent role in the reestablishment of brain osmotic equilibrium.
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