These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Decreased accumulation of glutelin types in rice grains constitutively expressing a sunflower seed albumin gene.
    Author: Islam N, Upadhyaya NM, Campbell PM, Akhurst R, Hagan N, Higgins TJ.
    Journal: Phytochemistry; 2005 Nov; 66(21):2534-9. PubMed ID: 16242165.
    Abstract:
    Previous studies have shown differential accumulation of sulfur rich glutelins and sulfur poor prolamins in transgenic rice seeds expressing a sunflower seed albumin gene [Hagan, N.D., Upadhyaya, N., Tabe, L.M., Higgins, T.J., 2003. The redistribution of protein sulfur in transgenic rice expressing a gene for a foreign, sulfur-rich protein. Plant J 34, 1-11]. Here, we show, by two-dimensional electrophoresis, differential accumulation of three classes of glutelin proteins - type I, II and III - and a globulin, not previously resolved, in transgenic seeds grown under low and high sulfur nutrition. Several glutelin polypeptides were resolved and four identified as a type I glutelin, two type II glutelins and a type III glutelin. Although sulfur nutrition did not affect the accumulation of sunflower seed albumin, the levels of all four identified glutelins and the globulin were lower in mature seeds derived from transgenic plants grown under sulfur-optimum or sulfur limited conditions compared to non-transgenic rice seeds. The reduction of all four glutelin polypeptides and the globulin varied from 21% to 68%. The re-allocation of sulfur reserves from endogenous proteins to the sulfur sink in transgenic grain is suggestive of a transcriptional control of sulfur mobilization in plants.
    [Abstract] [Full Text] [Related] [New Search]