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  • Title: Ionization of unconjugated, glycine- and taurine-conjugated bile acids by electrospray ionization mass spectrometry.
    Author: Mano N, Mori M, Ando M, Goto T, Goto J.
    Journal: J Pharm Biomed Anal; 2006 Mar 18; 40(5):1231-4. PubMed ID: 16242893.
    Abstract:
    We investigated the effect of organic anions as spray liquid additives on the ionization efficiency of unconjugated, glycine-conjugated and taurine-conjugated bile acids under electrospray ionization conditions. Addition of organic acids influenced the ionization efficiency of whole bile acids. Use of a stronger acid reduced the peak intensity of unconjugated and glycine-conjugated bile acids, while the use of TFA, the strongest acid tested, improved the intensity of taurine conjugates. The hydroxyl group at the C-12 alpha position of cholic acid and deoxycholic acid easily underwent intra-molecular hydrogen bonding with the side chain carboxyl group, accelerating the ionization efficiency. This intra-molecular hydrogen bond may also affect the formation of product ions in low energy-CID. The addition of ammonium ions to the spray liquid influenced the ionization of all bile acids, specifically enhancing the ionization efficiency of unconjugated bile acids.
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