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  • Title: Application of sequential integration for metabolic engineering of 1,2-propanediol production in yeast.
    Author: Lee W, Dasilva NA.
    Journal: Metab Eng; 2006 Jan; 8(1):58-65. PubMed ID: 16242982.
    Abstract:
    The yeast species Saccharomyces cerevisiae was engineered to produce 1,2-propanediol (1,2-PD) using the delta/UB sequential gene integration method. To study the effects of increased copy number, 2 genes (mgs and gldA from Escherichia coli) were sequentially integrated into the chromosomes of S. cerevisiae strains of opposite mating type. The resulting strains (containing 0-3 copies of either mgs or gldA) were mated to create all possible combinations of the 2 genes introduced for 1,2-PD production. Enzyme activities were generally correlated with copy number, although there was greater variation in GldA activity in the diploid cells. The integrated genes were confirmed by Southern blot and 1,2-PD production was analyzed by HPLC. The strain containing 3 copies of mgs and gldA showed the highest level of 1,2-PD; however, 1,2-PD concentration was not clearly related to gene copy number. 1,2-PD production did correlate with Mgs specific activity, and high GldA specific activity was found to be inhibitory.
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