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  • Title: [Immunogenicity of envelope glycoprotein gene of reticuloendotheliosis virus expressed in insect cell].
    Author: Wang XL, Zhang Z, Jiang SJ, Cui ZZ.
    Journal: Wei Sheng Wu Xue Bao; 2005 Aug; 45(4):593-7. PubMed ID: 16245878.
    Abstract:
    A recombinant baculovirus expressing reticuloendotheliosis virus env gene was constructed with Bac-to-Bac Baculovirus Expression Systems. After transfecting the recombinant virus into Sf9 cells for 3 days, REV env can be detected by indirect immunofluorescence antibody assay (IFA) and Western blot with specific monoclonal antibodies of REV. The oil-water emulsion vaccine was then produced using this infected Sf9 cells lycates and inoculated SPF chickens to validate the immunogenicity for REV. The results show that special anti-REV antibody can maintain more than 45 days and resist the infection of REV viruses. This is the first success to induce anti-REV antibody in chickens by none-live viruses.
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