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  • Title: [Immobilization of laccase on magnetic chitosan microspheres and study on its enzymic properties].
    Author: Jiang DS, Long SY, Huang J, Xiao HY, Zhou JY.
    Journal: Wei Sheng Wu Xue Bao; 2005 Aug; 45(4):630-3. PubMed ID: 16245887.
    Abstract:
    Laccase was immobilized on magnetic chitosan microspheres by using glutaraldehyde as cross-linking reagent. The immobilization conditions and characterization of the immobilized enzyme were investigated. immobilization conditions for laccase were: 10mL of 0.8mg/mL of laccase in phosphate buffer(0.1mol/L, pH 7.0) reacted with 50mg of magnetic chitosan microspheres at 25 degrees C for 1h and subsequently was kept at 4 degrees C for 2h. The immobilized enzyme exhibited the maximal activity at pH 3.0. The optimal temperatures for immobilized enzyme were 10 degrees C and 55 degrees C. The Km value of immobilized laccase for ABTS was 171.1 micromol/L in pH 3.0 phosphate buffer at 37 degrees C. Compared with free enzyme, the thermal, operational, and storage stabilities of the enzyme were increased after the immobilization.
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