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  • Title: Optimization of DsRed production in Escherichia coli: effect of ribosome binding site sequestration on translation efficiency.
    Author: Pfleger BF, Fawzi NJ, Keasling JD.
    Journal: Biotechnol Bioeng; 2005 Dec 05; 92(5):553-8. PubMed ID: 16247774.
    Abstract:
    DsRed-Express, a popular reporter protein, cannot be expressed in Escherichia coli using a consensus ribosome binding site (RBS) potentially due to basepairing in the RBS that inhibits translation initiation. Saturation mutagenesis was used to probe for a gene sequence that minimized basepairing in the RBS while maintaining the same spectral properties and maturation characteristics as DsRed-Express. The new DsRed, designated here as RFP(EC) for E. coli optimized red fluorescent protein, fluoresces 2.5 times greater than DsRed-Express when expressed from the same vector.
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