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  • Title: [A soft poration of planar bilayer lipid membranes from dipalmitoylphosphatidylcholine at the temperature of the phase transition from the liquid crystalline to the gel state].
    Author: Antonov VF, Anosov AA, Norik VP, Smirnova EIu.
    Journal: Biofizika; 2005; 50(5):867-77. PubMed ID: 16248162.
    Abstract:
    A method of soft poration of lipid bilayer was suggested, which is based on the structural rearrangement of lipid bilayer formed from disaturated phospholipids on the phase transition from liquid crystalline state to the gel. As opposed to the widely used method of electropbration, this method allows one to obtain a lipid pore population without application of high electric field. In the case of soft poration, the electric field does not exceed the physiological level of 10-100 mV. It was shown that, in planar bilayer lipid membranes formed from dipalmitoylphosphatidylcholine in water solution of 1 M LiCl, there appear up to 10 lipid pores in 1 min per 1 mm of membrane surface with an average conductivity of a pore of 31 +/- 13 nS. The average pore radius estimated using soluble polyethylene glycols ranged between 1.05-1.63 nm. Monovalent cation conductivity of a single lipid pore on soft poration was shown to decrease in the order Li+ > or = Na+ > K+ = Rb+ > or = Cs+. This order coincides with that observed by Marra and Israilashvili for dipalmitoylphosphatidylcholine-water interbilayer where the repulsive hydration force contribution is significant.
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