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  • Title: [Determination of amino acids in fu fang banlangen granulae by a pre-column derivatization RP-HPLC method].
    Author: Wan S, Xu M, Wang L, Kang T, Zhao C.
    Journal: Zhong Yao Cai; 2005 Jul; 28(7):594-6. PubMed ID: 16252729.
    Abstract:
    OBJECTIVE: To establish a pre-column derivatization reversed-phase high-performance liquid chromatographic method for the determination of amino acids, which was known as the characteristic constituents of Fu Fang Banlangen Granulae. METHODS: We determined the amino acids in alkalescence by a pre-column derivatization of DNFB. NaAc buffer (pH 6.4)-Acetonitrile (850:150) was used as the mobile phase on a SinoChrom ODS-BP column. The detector was operated at 360 nm. RESULTS: The method was carried out over the range of 0.627 - 5. 016 microg for Arg and 0.874 - 7.000 microg for Pro. The recoveries were 97.7% with the RSD = 2.0% for Arg and 97.7% with the RSD = 1.5% for Pro. CONCLUSIONS: The method has good accuracy and repeatability and it can be used for the quality control of Fu Fang Banlangen Granulae.
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