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  • Title: Analysis of gene-expression profiles after gamma irradiation of normal human fibroblasts.
    Author: Tachiiri S, Katagiri T, Tsunoda T, Oya N, Hiraoka M, Nakamura Y.
    Journal: Int J Radiat Oncol Biol Phys; 2006 Jan 01; 64(1):272-9. PubMed ID: 16257130.
    Abstract:
    PURPOSE: To understand comprehensive transcriptional profile of normal human fibroblast in response to irradiation. METHODS AND MATERIALS: To identify genes whose expression is influenced by gamma radiation, we used a cDNA microarray to analyze expression of 23,000 genes in normal human fibroblasts at 7 timepoints (1, 3, 6, 12, 24, 48, and 72 hours) after 5 different doses (0.5, 2, 5, 15, and 50 Gy) of exposure. RESULTS: Among the genes that showed altered expression patterns, some were already known to be regulated by irradiation, for instance ODC, EGR1, FGF2, PCNA, PKC, and several p53-target genes, including p53DINP1, BTG2, GADD45, and MDM2. The time course of each dose showed that from 350 to 600 genes were affected as to their expression; induction profiles characteristic to each dose were demonstrated. Of the total identified, only 89 genes were up-regulated; the vast majority was down-regulated over the 72-hour time course. We identified 21 genes that were distinctly induced by irradiation; 11 of them were functionally known, and 6 of those were p53-target genes. CONCLUSIONS: The results underscored the complexity of the transcriptional responses to irradiation, and the data should serve as a basis for global characterization of radiation-regulated genes and pathways.
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