These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Real-time reverse transcription polymerase chain reaction assay to detect a broad range of feline calicivirus isolates.
    Author: Wilhelm S, Truyen U.
    Journal: J Virol Methods; 2006 Apr; 133(1):105-8. PubMed ID: 16266755.
    Abstract:
    This report describes a real-time reverse transcription polymerase chain reaction (RT-PCR) assay with SYBR Green targeting the VP2 (ORF 3) of feline caliciviruses. All of the 44 feline calicivirus isolates tested were detected, whereas neither feline herpesvirus, feline panleukopenia virus, feline immunodeficiency virus and feline leukemia virus nor other calicivirus like rabbit hemorrhagic disease virus and a canine calicivirus isolate showed specific amplification products. The sensitivity of the SYBR Green reaction was shown to be equivalent to 5 x 10(1) to 5 x 10(2) copies/reaction and the overall sensitivity equivalent to a feline calicivirus titer of 10(0.6) TCID(50)/100 microl in Crandell Reese Feline Kidney (CRFK) cells.
    [Abstract] [Full Text] [Related] [New Search]