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  • Title: Representational difference analysis and real-time PCR for strain-specific quantification of Lactobacillus sobrius sp. nov.
    Author: Konstantinov SR, Smidt H, de Vos WM.
    Journal: Appl Environ Microbiol; 2005 Nov; 71(11):7578-81. PubMed ID: 16269808.
    Abstract:
    Lactobacillus sobrius sp. nov., which was recently isolated from the intestine of weaning piglets, has potential probiotic properties. To follow the fate of L. sobrius strain 001T in dietary interventions, a novel and strain-specific quantitative detection procedure was developed. This procedure was based on the isolation of specific genomic fragments from the type strain by representational difference analysis and their detection by real-time PCR. The described strain-specific quantification approach may be used in studies aimed at tracking bacterial strains added to specific environments.
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