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  • Title: [Purification and identification of surfactin isoforms produced by Bacillus subtilis B2 strain].
    Author: Gao X, Yao S, Huong P, Joachim V, Wang J.
    Journal: Wei Sheng Wu Xue Bao; 2003 Oct; 43(5):647-52. PubMed ID: 16281564.
    Abstract:
    Crude lipopeptide was extracted with methanol from the pellet which was precipitated with 6 mol/L HCl from the culture medium of Bacillus subtilis B2 strain. The crude lipopeptide was purified by gel filtration on Sephadex LH-20 column, the obtained product was referred to as sub-pure lipopeptide. The MALDI-TOF mass spectrum showed that B2 produced only lipopeptide surfactin. Sub-pure lipopeptide was run on muRPC C2/C18 column by HPLC SMART SYSTEM with aim at separation of surfactin isoforms. The surfactin isoforms fractions was detected by MALDI-TOF-PSD mass spectrometry, results indicated that the surfactin isoforms of B2 strain was cyclic lipodecapeptide L-Glu-L-Leu-D-Leu-L-Val-L-Asp-D-Leu-L-Leu containing fatty acid with a side chain length of 13, 14 and 15 carbon atoms.
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