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  • Title: Characteristics of (+)-catechin and (-)-epicatechin transport across pig intestinal brush border membranes.
    Author: Starp C, Alteheld B, Stehle P.
    Journal: Ann Nutr Metab; 2006; 50(1):59-65. PubMed ID: 16282679.
    Abstract:
    BACKGROUND/AIMS: (+)-Catechin and (-)-epicatechin are considered as disease preventive flavan-3-ols of foods like fruits, beverages and chocolate. We investigated mechanisms and kinetics of (+)-catechin and (-)-epicatechin uptake employing a validated in vitro model with isolated pig brush border membrane vesicles. METHODS: Vesicles were isolated from pig small intestine employing the divalent cation method. Characterization (marker enzymes, electron microscopy) confirmed their purity and function. Transport studies with (+)-catechin and (-)-epicatechin under predefined conditions [presence/absence of sodium, pH gradient, temperature (8-37 degrees C), various initial substrate concentrations (2-20 mmol/l)] revealed a measurable transport (HPLC analyses) across the brush border membrane for both substrates. RESULTS: Catechin transport was stimulated by an outwardly directed H(+) gradient (pH(i) 5.5/pH(o) 7.5). The presence of an inwardly directed Na(+) gradient did not result in a transient overshoot in (+)-catechin and (-)-epicatechin uptake. At 37 degrees C, subtraction of diffusion from the total transport rate showed saturation kinetics. CONCLUSION: Our in vitro study indicate that both (+)-catechin and (-)-epicatechin are transported across the basolateral membrane using a dual transport system consisting of free diffusion (dominant at low concentrations) and carrier-mediated facilitated diffusion.
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