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  • Title: Effect of phospholipase C induced hydrolysis of phospholipids on membrane-bound and water-soluble LH/hCG receptors in porcine corpora lutea.
    Author: Kolena J.
    Journal: Exp Clin Endocrinol; 1992; 99(1):8-11. PubMed ID: 1628700.
    Abstract:
    The role of phospholipids in the function of LH/hCG receptors was studied in two receptor preparations: the membrane fraction of porcine corpora lutea (CL) and the water-soluble receptor in follicular fluid (LFF) which was characterized. Digestion of CL membranes with phospholipase C (PL-C) abolished, in a dose responsive manner, specific binding of [125I]hCG and decreased phospholipid concentrations in the membranes. This loss of LH/hCG receptors was prevented by o-phenanthroline, an inhibitor of PL-C. A similar effect on membrane-bound receptors was observed when lipids were extracted with ethanol-diethylether. On the other hand, treatment of water-soluble receptors with PL-C or delipidation of LFF with Amberlit IRA 400 had no effect on [125I]hCG specific binding. These data suggest that phospholipids play an important role in the accessibility of membrane-bound receptors but are not involved in direct interaction of gonadotropin with binding sites.
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