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  • Title: Determination of ammonium in river water and sewage samples by capillary zone electrophoresis with direct UV detection.
    Author: Fukushi K, Ito H, Kimura K, Yokota K, Saito K, Chayama K, Takeda S, Wakida S.
    Journal: J Chromatogr A; 2006 Feb 17; 1106(1-2):61-6. PubMed ID: 16297395.
    Abstract:
    We developed capillary zone electrophoresis (CZE) with direct UV detection for determination of ammonium in environmental water samples. Ammonium in the samples was partly converted into ammonia in the alkaline background electrolyte (BGE) during migration and was detected by molecular absorption of ammonia at 190 nm in approximately 7 min. The limit of detection (LOD) for ammonium was 0.24 mg/l (as nitrogen) at a signal-to-noise ratio of three. The respective values of the relative standard deviation (RSD) of peak area, peak height, and migration time for ammonium were 2.1, 1.8, and 0.46%. Major alkali and alkaline earth metal ions coexisting in the samples did not interfere with ammonium determination by the proposed method. The proposed method determined ammonium in surface water and sewage samples. The results were compared to those obtained using ion chromatography (IC).
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