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  • Title: Estimation and separation of N-acetyl-beta-D-glucosaminidase isoenzymes in urine.
    Author: Javed MH, Hussain AN, Waqar MA.
    Journal: J Pak Med Assoc; 1992 Mar; 42(3):64-6. PubMed ID: 1630001.
    Abstract:
    N-acetyl-beta-D-glucosaminidase is a lysosomal enzyme made up of two isoenzymes (A and B). It has been used extensively as a marker for kidney damage. However, its estimation in urine has not been standardized. We have established a method for the estimation and separation of NAG isoenzymes by ion-exchange chromatography. In 19 experiments done so far, this method has given reproducible results. The significance of this method is that with a single experiment, one can estimate total as well as individual isoenzyme activity. Furthermore, urine constituents do not appear to interfere in this assay.
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