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  • Title: T-DNA tagging and characterization of a cryptic root-specific promoter in Arabidopsis.
    Author: Sivanandan C, Sujatha TP, Prasad AM, Resminath R, Thakare DR, Bhat SR, Srinivasan.
    Journal: Biochim Biophys Acta; 2005 Dec 20; 1731(3):202-8. PubMed ID: 16307804.
    Abstract:
    From a T-DNA tagged Arabidopsis population, a line, M-57 showing GUS (beta-glucuronidase) expression in the vascular regions of young roots was identified. Southern analysis revealed presence of a single T-DNA insert. Using inverse PCR, the plant sequence flanking the T-DNA insertion was cloned. The insertion was identified to be in the intergenic area between loci At4G13940 and At4G13930, coding for SAHH (S-Adenosyl-l-Homocysteine Hydrolase) and SHMT (Serine Hydroxy Methyl Transferase) genes, respectively. A 452-bp fragment immediately upstream of the T-DNA insertion when cloned and mobilized as a GUS fusion was capable of driving a similar root-specific expression of reporter gene in transgenic Arabidopsis plants and their progenies. This cryptic promoter element does not show the presence of any known root-specific promoter element.
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