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  • Title: [Development of Mycobacterium lacticolum and its synthesis of exopolysaccharide under conditions of varying acidity of the medium].
    Author: Gogoleva EV, Grechushkina NN, Egorov NS.
    Journal: Mikrobiologiia; 1975; 44(5):828-31. PubMed ID: 1632.
    Abstract:
    Mycobacterium lacticolum 121 grows and synthesizes exopolysaccharide at the initial values of pH of the medium from 5.0 to 11.5. The accumulation of biomass is maximal (10 to 12 gram/litre) at pH 6.0 to 11.5; the rate of growth is highest at pH 8.0 to 11.5. The largest amount of exopolysaccharide (2.0 to 2.4 gram/litre) is produced in the medium at pH 8.0 to 11.0; the rate of its production is highest at pH 9.0 to 11.0. The growth of Mycobacterium lacticolum 121 and the biosynthesis of polysaccharide are optimal at pH 8.0 to 8.2. Changes in acidity of the medium have no effect on the qualitative composition, structure, and molecular weight of the polysaccharide.
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