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  • Title: Phosphorylation of glutathione-S-transferase by protein kinase C-alpha implications for affinity-tag purification.
    Author: Rodriguez P, Mitton B, Kranias EG.
    Journal: Biotechnol Lett; 2005 Dec; 27(23-24):1869-73. PubMed ID: 16328982.
    Abstract:
    Expression and purification of proteins as fusions with glutathione S-transferase (GST) is a standard and widely employed system. In more than 2,500 published studies, GST has been used to facilitate the purification of recombinant proteins, assess protein-protein interactions, and establish protein function. In this report, we provide evidence that GST can be phosphorylated in vitro by protein kinase C-alpha (PKC-alpha) at Ser-93. Therefore, since GST itself may be a target for a number of catalytic enzymes, failure to remove the GST tag from the recombinant protein may lead to inaccurate conclusions.
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