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  • Title: Salt is necessary for nucleosomal DNA fragmentation induced by caspase.
    Author: Kim BE, Lee YH, Seong C, Shin SY, Lee JS, Cho BY, Park IS.
    Journal: Biotechnol Lett; 2005 Dec; 27(23-24):1935-42. PubMed ID: 16328993.
    Abstract:
    For nucleosomal DNA fragmentation, one of the hallmarks of apoptosis, activated caspase, an apoptosis specific cysteine protease, is required to cleave ICAD/DFF45 that releases its complexed DNase, CAD/DFF40. The protein complex is located predominantly in the nuclei. Inconsistently, caspase alone cannot induce DNA fragmentation in the isolated nuclei without the addition of a cell extract or purified CAD/DFF40. In this study, however, it is demonstrated that under selected conditions with 50-75 mM: KCl or NaCl, caspase-3 and-7 can induce DNA fragmentation without the additional factor(s).
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