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Title: Application of cation-exchange membranes for characterisation and imaging ammonia-oxidising bacteria in soils. Author: Kurola J, Wittmann C, Salkinoja-Salonen M, Aarnio T, Romantschuk M. Journal: FEMS Microbiol Ecol; 2005 Aug 01; 53(3):463-72. PubMed ID: 16329964. Abstract: A new approach, in which ammonia-oxidizing bacteria (AOB) are entrapped from soil onto cation-exchange membranes, was applied to identify terrestrial AOB by fluorescence in situ hybridization (FISH). An experimental hot spot of ammonia oxidation was developed by establishing a gradient of ammonium substrate (200 to <20 mg NH4+-N l(-1)) diffused through the cation-exchange membranes incubated in soil for 6 months. By this approach we were able to characterise and image indigenous AOB populations growing in heavily oil-polluted soil using FISH and sequence analysis of PCR-amplified 16S rRNA genes, respectively. The FISH results revealed that Nitrosospira-like AOB were dominant on the ammonium-enriched membranes incubated in the soil. Fourteen unique Nitrosospira-like 16S rRNA gene sequences belonging to clusters 2 and 3 were recovered from the soil-incubated membranes and from the soil, suggesting the importance of Nitrosospira-like AOB in the oil-polluted landfarming soil.[Abstract] [Full Text] [Related] [New Search]