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  • Title: Plasmids for nicotine-dependent and -independent gene expression in Arthrobacter nicotinovorans and other arthrobacter species.
    Author: Sandu C, Chiribau CB, Sachelaru P, Brandsch R.
    Journal: Appl Environ Microbiol; 2005 Dec; 71(12):8920-4. PubMed ID: 16332890.
    Abstract:
    The first inducible Arthrobacter overexpression system, based on the promoter/operator and the repressor of the 6-D-hydroxynicotine oxidase gene of Arthrobacter nicotinovorans, is described here. Nicotine-dependent overproduction and affinity purification of recombinant proteins are presented. The system will allow the production of complex enzymes and genetic complementation studies in Arthrobacter species.
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