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  • Title: Development of a triplex real-time PCR assay for detection of Panton-Valentine leukocidin toxin genes in clinical isolates of methicillin-resistant Staphylococcus aureus.
    Author: McDonald RR, Antonishyn NA, Hansen T, Snook LA, Nagle E, Mulvey MR, Levett PN, Horsman GB.
    Journal: J Clin Microbiol; 2005 Dec; 43(12):6147-9. PubMed ID: 16333116.
    Abstract:
    Community-associated methicillin-resistant Staphylococcus aureus harboring Panton-Valentine leukocidin (PVL) genes is an emerging pathogen. A novel real-time PCR assay for identification of MRSA isolates containing PVL was developed. The PVL assay was used in a triplex format allowing simultaneous amplification of mecA, nuc, and PVL genes in 614 clinical isolates. This assay facilitates the rapid identification of PVL-positive isolates of MRSA.
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