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  • Title: Transcripts of the viroid central conserved region contain the local tertiary structural element found in full-length viroid.
    Author: Paul CP, Levine BJ, Robertson HD, Branch AD.
    Journal: FEBS Lett; 1992 Jun 22; 305(1):9-14. PubMed ID: 1633862.
    Abstract:
    The viroid central conserved region (CCR) is highly conserved among different viroids and is thought to be involved in viroid replication. A novel tertiary structure occurs in the CCR of native circular potato spindle tuber RNAs. To permit more detailed studies of this structural element, a small RNA oligonucleotide containing the CCR of the viroid genome was synthesized. The tertiary structure of these CCR transcripts was examined by UV-crosslinking of the RNA, followed by mapping of the crosslink using limited alkaline digestion and classical RNA secondary analysis. The CCR transcript was found to undergo UV-crosslinking between the same two bases as in full-length viroid, indicating that the tertiary structure is the same and that the CCR transcript will be useful for the affinity purification of host components.
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