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  • Title: The metabolic profiles of transgenic cucumber lines vary with different chromosomal locations of the transgene.
    Author: Tagashira N, Plader W, Filipecki M, Yin Z, Wiśniewska A, Gaj P, Szwacka M, Fiehn O, Hoshi Y, Kondo K, Malepszy S.
    Journal: Cell Mol Biol Lett; 2005; 10(4):697-710. PubMed ID: 16341278.
    Abstract:
    The metabolic profiles of five transgenic cucumber lines were compared taking into consideration their transgene integration sites. The plants analyzed were homozygous and contained transgenes integrated in a single locus on chromosomes I, II, III or IV. The transgenes were preferentially located in the euchromatic regions. Each of these locations possessed a specific metabolic profile. The number of altered compounds in the transgenic lines varied between 9 and 23 of the 47 metabolites identified. These alterations seem to be specific for each independent transgene integration. However, some changes are common: a decrease in the levels of phenylalanine, aspartate, ethanolamine and pipecolate, and an increase in the level of benzoic acid. The observed effects of transgene introduction are discussed in this paper.
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