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  • Title: [Determination of fibronectin-binding region of FBPS of Streptococcus suis type 2].
    Author: Sun LY, Fan HJ, Lu CP.
    Journal: Wei Sheng Wu Xue Bao; 2005 Oct; 45(5):753-6. PubMed ID: 16342770.
    Abstract:
    Fusion expression plasmid pFBPS, pFBPS (7 - 82), pFBPS (7 - 165) and pFBPS3 (87 - 320) of Streptococcus suis type strain HA9801 were generated by cloning different fragments of fbps amplified from pMD-T-FBPS by PCR into plasmid pET-32a(+). It has been confirmed that the recombinant proteins, FBPS, FBPS(87 -320), and FBPS(7 - 167), which are expressed by recombinant plasmid pFBPS, pFBPS(87 - 320) and pFBPS(7 - 165), respectively, bound human fibronectin by ligand affinity Western blot assay. The results indicate the primary fibronectin-binding domain of FBPS lies within 87 - 165 amino acid residues region.
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