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  • Title: Penetration of Rhizopus oligosporus into Soybeans in Tempeh.
    Author: Jurus AM, Sundberg WJ.
    Journal: Appl Environ Microbiol; 1976 Aug; 32(2):284-7. PubMed ID: 16345170.
    Abstract:
    Histological observations were made on the penetration of hyphae of Rhizopus oligosporus into soybean cotyledons in tempeh, an Indonesian soybean food. Hyphal penetrations averaged one per 1,400 mum (+/-390 mum) on the curved (outer) cotyledon surface and one per 1,010 mum (+/-340 mum) on the flat (inner) one. Hyphae infiltrated to a depth of 742 mum, or about 25% of the average width of a soybean cotyledon. This previously unreported degree of penetration offers partial explanation for the rapid physical and chemical changes in soybeans during tempeh fermentation.
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