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  • Title: Electron spin resonance for detecting polyadenylate tracts in RNA's.
    Author: Bobst AM, Sinha TK, Pan YE.
    Journal: Science; 1975 Apr 11; 188(4184):153-5. PubMed ID: 163495.
    Abstract:
    Electron spin resonance is used to detect RNA's that contain polyadenylate tracts. The method depends on the ability of RNA's that contain polyadenylate sequences to associate with poly(2'-deoxy-2'-fluoro)uridylic acid, which has been spin-labeled with 4-(2-iodoacetamido)-2,2,6,6-tetramethylpiperidinooxyl. The formation of the hybridization product can be detected by monitoring the decrease in mobility of the spin probe.
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