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  • Title: Ultrastructural organization of Alicyclobacillus tolerans strain K1T cells.
    Author: Duda VI, Suzina NE, Severina LO, Bogdanova TI, Tsaplina IA, Karavaiko GI.
    Journal: Arch Microbiol; 2006 Mar; 185(1):63-8. PubMed ID: 16362286.
    Abstract:
    Electron microscopy examinations of thin sections and freeze-fracture replicas revealed the specific ultrastructural features of Alicyclobacillus tolerans strain K1(T). In particular, the cell wall displayed an ultrastructure typical of gram-positive bacteria and consisted of a thin murein layer (50-60 A in thickness); cells exhibited a surface S-layer constituted by large hexagonally packed (p6-symmetry) rod-shaped subunits of 150-160 A in diameter and 200 A in height. In the cytoplasmic membrane, there were intramembrane vesicular structures that sometimes appeared as large leaflets in the central part. The cytoplasm contained numerous vesicular inclusions covered with a monolayered wall, dissimilar to bilamellar lipid membranes. Endospore coats displayed an intricate structure and consisted of three thick layers; the outer layer had an unusual fine structure; the exosporium was also found.
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